The preparation of bovine collagen peptides is a multi-step process, mainly extracting collagen from bovine tissues such as skin, bone, tendons, etc., and then decomposing it into small molecule peptides through enzymatic hydrolysis or chemical treatment. The following is a typical preparation process:
- Raw material pretreatment
Source selection: commonly used cowhide, cow bone or cartilage (ensuring that the raw materials are free of diseases and meet food safety standards).
Cleaning and degreasing: removing impurities, fat, and non collagen components.
Demineralization treatment (for bone materials): Dissolve calcium in dilute acid to obtain a softened organic matrix. - Extraction of collagen protein
Hot water extraction: High temperature (60-90 ℃) denatures collagen to form gelatin (macromolecular collagen fragments). - Enzymatic hydrolysis process (key steps)
Enzyme selection: Use protease to selectively hydrolyze gelatin and cleave the peptide bonds of collagen.
Control conditions: Adjust temperature (40-60 ℃), pH (depending on enzyme type), and time (several hours to tens of hours) to avoid excessive hydrolysis.
Enzyme inactivation: Heating inactivates the enzyme and terminates the reaction. - Purification and concentration
Filtration: Remove unhydrolyzed large molecular impurities, commonly using ultrafiltration membranes to screen by molecular weight (such as 1-10 kDa).
Decolorization and deodorization: Activated carbon adsorption or ion exchange resin removes odors and pigments.
Nanofiltration: desalination, removal of impurities
Concentrated drying: spray drying or freeze drying to obtain powdered collagen peptide. - Quality control
Molecular weight detection: HPLC or mass spectrometry to confirm peptide distribution (usually targeting 500-3000 daltons).
Amino acid analysis: detect the content of glycine, proline, and hydroxyproline (characteristic amino acids of collagen).
Safety testing: Heavy metals, microorganisms, and other indicators must comply with food or drug standards.
Precautions
Raw material safety: It is necessary to avoid the risk of mad cow disease (BSE) and ensure reliable sources.
Process optimization: Enzymatic hydrolysis conditions directly affect the biological activity of peptides, such as antioxidant and reparative functions.
Application direction: Different uses such as food, health products (such as oral beauty), medical dressings, etc. have different purity requirements.
Through the above process, bovine collagen peptides can be made into easily absorbable small molecule forms, combining the nutritional value of collagen with the physiological activity of peptides.
Chenkang collagen peptide raw materials are strictly controlled and produced using advanced production processes with multiple inspections, making them trustworthy.